An in vitro limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse
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چکیده
منابع مشابه
An in vitro limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse.
We have developed a limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse using a miniturized stroma-dependent bone marrow culture assay in vitro. The cells were overlaid on irradiated stromal layers in microtiter wells in a range of concentrations, and frequencies of cobblestone area-forming cells (CAFC) were calculated by employing Poisson statistics. The pro...
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ژورنال
عنوان ژورنال: Blood
سال: 1989
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v74.8.2755.bloodjournal7482755